Highlights of PURIMEX DNA synthesis:

  • Very fast delivery times
  • Guaranteed delivery amounts to 50 bases
  • Chemicals from reputable manufacturers
  • Control of production by HPLC and PAGE
  • HPLC purification on a chain length of 35 bases
  • Using synthesizers of the company. Applied Biosystems
  • Optimized, not designed for thriftiness coupling cycles
  • Online monitoring of coupling efficiency by electrochemical detection
  • Oligo is desalted, measured and adjusted to a concentration of 100 uM

The guaranteed delivery volumes as a function of purification up to a length of 50 bases shown in the following table.When oligonucleotides that are longer than 70 bases, each half is provided.

scale

[nmol]

FAD [nmol]

desalted

1x HPLC

2x HPLC

3x HPLC

4x HPLC

5x HPLC

PAGE

10

2

1.2

1

-

-

-

-

50

10

6

5

4

3.5

3

2

200

50

30

25

20

17.5

15

10

1000

200

120

100

80

70

60

50

The following minimum standards, depending on the chain length must be ordered:

Chain length

Minimum scale [nmol]

10-25

10

26-50

50

51-100

200

> 100

1000 High Quality

designation

Price [euro] / clutch

10 nmol

50 nmol

200 nmol

1000 nmol

Standard DNA clutch

0.30

0.35

0.70

1.40

High Quality DNA oligonucleotides

Oligonucleotides are made of the special purity requirements or oligonucleotides that are longer than 100 bases are prepared using specific carrier materials and synthesis cycles. The crude product is (not included, see purification) as the standard DNA synthesis HPLC purified, measured and adjusted. Fixed delivery volumes can be guaranteed for oligos longer than 50 bases, not.

designation

Price [euro] / clutch

50 nmol

200 nmol

1000 nmol

High Quality DNA

0.70

1.00

2.00

Wobbles are manufactured exclusively by manual, accurate pre-mix of the bases. For each mixture, a "bottle setup" is calculated. Automatic mix on synthesizer at no extra charge, we have not for reasons of accuracy.

designation

Price [EUR]

Bottle setup

10

Setup 3'-CPG

10

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